anti dsdna antibodies Search Results


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Anti Dsdna, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 3. ApoExos trigger the production of autoantibodies. (a) <t>ELISA</t> was performed on the sera from ApoExos- or vehicle-injected WT mice (n = 5), and a significant increase of circulating total IgG was observed (p=0.0481). (b) Microarray experiments of multiple autoantigens showed an SLE-associated autoantibody profile in mice injected with ApoExos, but not in those injected with vehicle. Autoantibodies detected were IgG. Each row of the heatmap represents one animal for a total of 12 ApoExos-injected WT mice and 10 vehicle-injected WT mice. The results were compared by ANOVA and minimal q value of represented data was 0,05. Blue and yellow are low and high level respectively. ELISA of sera from ApoExos- or vehicle-injected mice confirmed the increased levels of SLE-associated autoantibodies (i.e., (c) anti-LG3 (p=0.0019), (d) antinuclear antibodies (ANA) (p=0.0009), (e) anti-dsDNA (p=0.0212), (f) anti-Sm/nRNP (p=0.0124), (g) anti-Sm (p=0.0008), (h) anti- SSA (Ro) (p=0.0195), (i) anti-SSB (La) (p=0.0002), (j) anti-cardiolipin (CL) (p=0.0001) and (k) anti-β2GPI (p<0.0001)), while autoantibodies related to autoim munity in transplantation were not affected (i.e., (l) anti-AT1R, (m) anti-vimentin and (n) anti-fibronectin). Statistical significance was assessed by Student’s t-test. ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05; ns not significant. Results are expressed as indicated: AU, Arbitrary Units; OD, Optical Density. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
Anti Dsdna Mouse Elisa Kits, supplied by BioVendor Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 3. ApoExos trigger the production of autoantibodies. (a) <t>ELISA</t> was performed on the sera from ApoExos- or vehicle-injected WT mice (n = 5), and a significant increase of circulating total IgG was observed (p=0.0481). (b) Microarray experiments of multiple autoantigens showed an SLE-associated autoantibody profile in mice injected with ApoExos, but not in those injected with vehicle. Autoantibodies detected were IgG. Each row of the heatmap represents one animal for a total of 12 ApoExos-injected WT mice and 10 vehicle-injected WT mice. The results were compared by ANOVA and minimal q value of represented data was 0,05. Blue and yellow are low and high level respectively. ELISA of sera from ApoExos- or vehicle-injected mice confirmed the increased levels of SLE-associated autoantibodies (i.e., (c) anti-LG3 (p=0.0019), (d) antinuclear antibodies (ANA) (p=0.0009), (e) anti-dsDNA (p=0.0212), (f) anti-Sm/nRNP (p=0.0124), (g) anti-Sm (p=0.0008), (h) anti- SSA (Ro) (p=0.0195), (i) anti-SSB (La) (p=0.0002), (j) anti-cardiolipin (CL) (p=0.0001) and (k) anti-β2GPI (p<0.0001)), while autoantibodies related to autoim munity in transplantation were not affected (i.e., (l) anti-AT1R, (m) anti-vimentin and (n) anti-fibronectin). Statistical significance was assessed by Student’s t-test. ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05; ns not significant. Results are expressed as indicated: AU, Arbitrary Units; OD, Optical Density. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
Sku Dsd31 K01, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 3. ApoExos trigger the production of autoantibodies. (a) <t>ELISA</t> was performed on the sera from ApoExos- or vehicle-injected WT mice (n = 5), and a significant increase of circulating total IgG was observed (p=0.0481). (b) Microarray experiments of multiple autoantigens showed an SLE-associated autoantibody profile in mice injected with ApoExos, but not in those injected with vehicle. Autoantibodies detected were IgG. Each row of the heatmap represents one animal for a total of 12 ApoExos-injected WT mice and 10 vehicle-injected WT mice. The results were compared by ANOVA and minimal q value of represented data was 0,05. Blue and yellow are low and high level respectively. ELISA of sera from ApoExos- or vehicle-injected mice confirmed the increased levels of SLE-associated autoantibodies (i.e., (c) anti-LG3 (p=0.0019), (d) antinuclear antibodies (ANA) (p=0.0009), (e) anti-dsDNA (p=0.0212), (f) anti-Sm/nRNP (p=0.0124), (g) anti-Sm (p=0.0008), (h) anti- SSA (Ro) (p=0.0195), (i) anti-SSB (La) (p=0.0002), (j) anti-cardiolipin (CL) (p=0.0001) and (k) anti-β2GPI (p<0.0001)), while autoantibodies related to autoim munity in transplantation were not affected (i.e., (l) anti-AT1R, (m) anti-vimentin and (n) anti-fibronectin). Statistical significance was assessed by Student’s t-test. ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05; ns not significant. Results are expressed as indicated: AU, Arbitrary Units; OD, Optical Density. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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Fig. 3. ApoExos trigger the production of autoantibodies. (a) <t>ELISA</t> was performed on the sera from ApoExos- or vehicle-injected WT mice (n = 5), and a significant increase of circulating total IgG was observed (p=0.0481). (b) Microarray experiments of multiple autoantigens showed an SLE-associated autoantibody profile in mice injected with ApoExos, but not in those injected with vehicle. Autoantibodies detected were IgG. Each row of the heatmap represents one animal for a total of 12 ApoExos-injected WT mice and 10 vehicle-injected WT mice. The results were compared by ANOVA and minimal q value of represented data was 0,05. Blue and yellow are low and high level respectively. ELISA of sera from ApoExos- or vehicle-injected mice confirmed the increased levels of SLE-associated autoantibodies (i.e., (c) anti-LG3 (p=0.0019), (d) antinuclear antibodies (ANA) (p=0.0009), (e) anti-dsDNA (p=0.0212), (f) anti-Sm/nRNP (p=0.0124), (g) anti-Sm (p=0.0008), (h) anti- SSA (Ro) (p=0.0195), (i) anti-SSB (La) (p=0.0002), (j) anti-cardiolipin (CL) (p=0.0001) and (k) anti-β2GPI (p<0.0001)), while autoantibodies related to autoim munity in transplantation were not affected (i.e., (l) anti-AT1R, (m) anti-vimentin and (n) anti-fibronectin). Statistical significance was assessed by Student’s t-test. ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05; ns not significant. Results are expressed as indicated: AU, Arbitrary Units; OD, Optical Density. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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Fig. 3. ApoExos trigger the production of autoantibodies. (a) <t>ELISA</t> was performed on the sera from ApoExos- or vehicle-injected WT mice (n = 5), and a significant increase of circulating total IgG was observed (p=0.0481). (b) Microarray experiments of multiple autoantigens showed an SLE-associated autoantibody profile in mice injected with ApoExos, but not in those injected with vehicle. Autoantibodies detected were IgG. Each row of the heatmap represents one animal for a total of 12 ApoExos-injected WT mice and 10 vehicle-injected WT mice. The results were compared by ANOVA and minimal q value of represented data was 0,05. Blue and yellow are low and high level respectively. ELISA of sera from ApoExos- or vehicle-injected mice confirmed the increased levels of SLE-associated autoantibodies (i.e., (c) anti-LG3 (p=0.0019), (d) antinuclear antibodies (ANA) (p=0.0009), (e) anti-dsDNA (p=0.0212), (f) anti-Sm/nRNP (p=0.0124), (g) anti-Sm (p=0.0008), (h) anti- SSA (Ro) (p=0.0195), (i) anti-SSB (La) (p=0.0002), (j) anti-cardiolipin (CL) (p=0.0001) and (k) anti-β2GPI (p<0.0001)), while autoantibodies related to autoim munity in transplantation were not affected (i.e., (l) anti-AT1R, (m) anti-vimentin and (n) anti-fibronectin). Statistical significance was assessed by Student’s t-test. ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05; ns not significant. Results are expressed as indicated: AU, Arbitrary Units; OD, Optical Density. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
Mouse Anti Dsdna Igm Antibody Assay Kit, supplied by Chondrex Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 3. ApoExos trigger the production of autoantibodies. (a) <t>ELISA</t> was performed on the sera from ApoExos- or vehicle-injected WT mice (n = 5), and a significant increase of circulating total IgG was observed (p=0.0481). (b) Microarray experiments of multiple autoantigens showed an SLE-associated autoantibody profile in mice injected with ApoExos, but not in those injected with vehicle. Autoantibodies detected were IgG. Each row of the heatmap represents one animal for a total of 12 ApoExos-injected WT mice and 10 vehicle-injected WT mice. The results were compared by ANOVA and minimal q value of represented data was 0,05. Blue and yellow are low and high level respectively. ELISA of sera from ApoExos- or vehicle-injected mice confirmed the increased levels of SLE-associated autoantibodies (i.e., (c) anti-LG3 (p=0.0019), (d) antinuclear antibodies (ANA) (p=0.0009), (e) anti-dsDNA (p=0.0212), (f) anti-Sm/nRNP (p=0.0124), (g) anti-Sm (p=0.0008), (h) anti- SSA (Ro) (p=0.0195), (i) anti-SSB (La) (p=0.0002), (j) anti-cardiolipin (CL) (p=0.0001) and (k) anti-β2GPI (p<0.0001)), while autoantibodies related to autoim munity in transplantation were not affected (i.e., (l) anti-AT1R, (m) anti-vimentin and (n) anti-fibronectin). Statistical significance was assessed by Student’s t-test. ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05; ns not significant. Results are expressed as indicated: AU, Arbitrary Units; OD, Optical Density. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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Fig. 3. ApoExos trigger the production of autoantibodies. (a) <t>ELISA</t> was performed on the sera from ApoExos- or vehicle-injected WT mice (n = 5), and a significant increase of circulating total IgG was observed (p=0.0481). (b) Microarray experiments of multiple autoantigens showed an SLE-associated autoantibody profile in mice injected with ApoExos, but not in those injected with vehicle. Autoantibodies detected were IgG. Each row of the heatmap represents one animal for a total of 12 ApoExos-injected WT mice and 10 vehicle-injected WT mice. The results were compared by ANOVA and minimal q value of represented data was 0,05. Blue and yellow are low and high level respectively. ELISA of sera from ApoExos- or vehicle-injected mice confirmed the increased levels of SLE-associated autoantibodies (i.e., (c) anti-LG3 (p=0.0019), (d) antinuclear antibodies (ANA) (p=0.0009), (e) anti-dsDNA (p=0.0212), (f) anti-Sm/nRNP (p=0.0124), (g) anti-Sm (p=0.0008), (h) anti- SSA (Ro) (p=0.0195), (i) anti-SSB (La) (p=0.0002), (j) anti-cardiolipin (CL) (p=0.0001) and (k) anti-β2GPI (p<0.0001)), while autoantibodies related to autoim munity in transplantation were not affected (i.e., (l) anti-AT1R, (m) anti-vimentin and (n) anti-fibronectin). Statistical significance was assessed by Student’s t-test. ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05; ns not significant. Results are expressed as indicated: AU, Arbitrary Units; OD, Optical Density. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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Fig. 3. ApoExos trigger the production of autoantibodies. (a) <t>ELISA</t> was performed on the sera from ApoExos- or vehicle-injected WT mice (n = 5), and a significant increase of circulating total IgG was observed (p=0.0481). (b) Microarray experiments of multiple autoantigens showed an SLE-associated autoantibody profile in mice injected with ApoExos, but not in those injected with vehicle. Autoantibodies detected were IgG. Each row of the heatmap represents one animal for a total of 12 ApoExos-injected WT mice and 10 vehicle-injected WT mice. The results were compared by ANOVA and minimal q value of represented data was 0,05. Blue and yellow are low and high level respectively. ELISA of sera from ApoExos- or vehicle-injected mice confirmed the increased levels of SLE-associated autoantibodies (i.e., (c) anti-LG3 (p=0.0019), (d) antinuclear antibodies (ANA) (p=0.0009), (e) anti-dsDNA (p=0.0212), (f) anti-Sm/nRNP (p=0.0124), (g) anti-Sm (p=0.0008), (h) anti- SSA (Ro) (p=0.0195), (i) anti-SSB (La) (p=0.0002), (j) anti-cardiolipin (CL) (p=0.0001) and (k) anti-β2GPI (p<0.0001)), while autoantibodies related to autoim munity in transplantation were not affected (i.e., (l) anti-AT1R, (m) anti-vimentin and (n) anti-fibronectin). Statistical significance was assessed by Student’s t-test. ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05; ns not significant. Results are expressed as indicated: AU, Arbitrary Units; OD, Optical Density. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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Fig. 3. ApoExos trigger the production of autoantibodies. (a) <t>ELISA</t> was performed on the sera from ApoExos- or vehicle-injected WT mice (n = 5), and a significant increase of circulating total IgG was observed (p=0.0481). (b) Microarray experiments of multiple autoantigens showed an SLE-associated autoantibody profile in mice injected with ApoExos, but not in those injected with vehicle. Autoantibodies detected were IgG. Each row of the heatmap represents one animal for a total of 12 ApoExos-injected WT mice and 10 vehicle-injected WT mice. The results were compared by ANOVA and minimal q value of represented data was 0,05. Blue and yellow are low and high level respectively. ELISA of sera from ApoExos- or vehicle-injected mice confirmed the increased levels of SLE-associated autoantibodies (i.e., (c) anti-LG3 (p=0.0019), (d) antinuclear antibodies (ANA) (p=0.0009), (e) anti-dsDNA (p=0.0212), (f) anti-Sm/nRNP (p=0.0124), (g) anti-Sm (p=0.0008), (h) anti- SSA (Ro) (p=0.0195), (i) anti-SSB (La) (p=0.0002), (j) anti-cardiolipin (CL) (p=0.0001) and (k) anti-β2GPI (p<0.0001)), while autoantibodies related to autoim munity in transplantation were not affected (i.e., (l) anti-AT1R, (m) anti-vimentin and (n) anti-fibronectin). Statistical significance was assessed by Student’s t-test. ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05; ns not significant. Results are expressed as indicated: AU, Arbitrary Units; OD, Optical Density. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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Image Search Results


Fig. 3. ApoExos trigger the production of autoantibodies. (a) ELISA was performed on the sera from ApoExos- or vehicle-injected WT mice (n = 5), and a significant increase of circulating total IgG was observed (p=0.0481). (b) Microarray experiments of multiple autoantigens showed an SLE-associated autoantibody profile in mice injected with ApoExos, but not in those injected with vehicle. Autoantibodies detected were IgG. Each row of the heatmap represents one animal for a total of 12 ApoExos-injected WT mice and 10 vehicle-injected WT mice. The results were compared by ANOVA and minimal q value of represented data was 0,05. Blue and yellow are low and high level respectively. ELISA of sera from ApoExos- or vehicle-injected mice confirmed the increased levels of SLE-associated autoantibodies (i.e., (c) anti-LG3 (p=0.0019), (d) antinuclear antibodies (ANA) (p=0.0009), (e) anti-dsDNA (p=0.0212), (f) anti-Sm/nRNP (p=0.0124), (g) anti-Sm (p=0.0008), (h) anti- SSA (Ro) (p=0.0195), (i) anti-SSB (La) (p=0.0002), (j) anti-cardiolipin (CL) (p=0.0001) and (k) anti-β2GPI (p<0.0001)), while autoantibodies related to autoim munity in transplantation were not affected (i.e., (l) anti-AT1R, (m) anti-vimentin and (n) anti-fibronectin). Statistical significance was assessed by Student’s t-test. ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05; ns not significant. Results are expressed as indicated: AU, Arbitrary Units; OD, Optical Density. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Journal: Journal of Translational Autoimmunity

Article Title: Vascular injury derived apoptotic exosome-like vesicles trigger autoimmunity

doi: 10.1016/j.jtauto.2024.100250

Figure Lengend Snippet: Fig. 3. ApoExos trigger the production of autoantibodies. (a) ELISA was performed on the sera from ApoExos- or vehicle-injected WT mice (n = 5), and a significant increase of circulating total IgG was observed (p=0.0481). (b) Microarray experiments of multiple autoantigens showed an SLE-associated autoantibody profile in mice injected with ApoExos, but not in those injected with vehicle. Autoantibodies detected were IgG. Each row of the heatmap represents one animal for a total of 12 ApoExos-injected WT mice and 10 vehicle-injected WT mice. The results were compared by ANOVA and minimal q value of represented data was 0,05. Blue and yellow are low and high level respectively. ELISA of sera from ApoExos- or vehicle-injected mice confirmed the increased levels of SLE-associated autoantibodies (i.e., (c) anti-LG3 (p=0.0019), (d) antinuclear antibodies (ANA) (p=0.0009), (e) anti-dsDNA (p=0.0212), (f) anti-Sm/nRNP (p=0.0124), (g) anti-Sm (p=0.0008), (h) anti- SSA (Ro) (p=0.0195), (i) anti-SSB (La) (p=0.0002), (j) anti-cardiolipin (CL) (p=0.0001) and (k) anti-β2GPI (p<0.0001)), while autoantibodies related to autoim munity in transplantation were not affected (i.e., (l) anti-AT1R, (m) anti-vimentin and (n) anti-fibronectin). Statistical significance was assessed by Student’s t-test. ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05; ns not significant. Results are expressed as indicated: AU, Arbitrary Units; OD, Optical Density. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Antinuclear antibodies (ANA) levels were assessed using ANA mouse bioassay kits (USBiologicals, Swampscott, MA, USA); total IgG levels were assessed with mouse IgG Total Ready-SET-Go kits (Affimetrix, Vienna, Austria); anti-double strand DNA (anti-dsDNA) levels were assessed with anti-dsDNA mouse ELISA kits (BioVendor, Neuss, Deutschland); and the levels of angiotensin 1 receptor antibodies (antiAT1R) were assessed with BioAssayTM ELISA Kits, mouse (USBiological, Salem, MA, USA), all in accordance with the manufacturers’ instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Injection, Microarray, Immunopeptidomics, Transplantation Assay