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Image Search Results
Journal: Journal of Translational Autoimmunity
Article Title: Vascular injury derived apoptotic exosome-like vesicles trigger autoimmunity
doi: 10.1016/j.jtauto.2024.100250
Figure Lengend Snippet: Fig. 3. ApoExos trigger the production of autoantibodies. (a) ELISA was performed on the sera from ApoExos- or vehicle-injected WT mice (n = 5), and a significant increase of circulating total IgG was observed (p=0.0481). (b) Microarray experiments of multiple autoantigens showed an SLE-associated autoantibody profile in mice injected with ApoExos, but not in those injected with vehicle. Autoantibodies detected were IgG. Each row of the heatmap represents one animal for a total of 12 ApoExos-injected WT mice and 10 vehicle-injected WT mice. The results were compared by ANOVA and minimal q value of represented data was 0,05. Blue and yellow are low and high level respectively. ELISA of sera from ApoExos- or vehicle-injected mice confirmed the increased levels of SLE-associated autoantibodies (i.e., (c) anti-LG3 (p=0.0019), (d) antinuclear antibodies (ANA) (p=0.0009), (e) anti-dsDNA (p=0.0212), (f) anti-Sm/nRNP (p=0.0124), (g) anti-Sm (p=0.0008), (h) anti- SSA (Ro) (p=0.0195), (i) anti-SSB (La) (p=0.0002), (j) anti-cardiolipin (CL) (p=0.0001) and (k) anti-β2GPI (p<0.0001)), while autoantibodies related to autoim munity in transplantation were not affected (i.e., (l) anti-AT1R, (m) anti-vimentin and (n) anti-fibronectin). Statistical significance was assessed by Student’s t-test. ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05; ns not significant. Results are expressed as indicated: AU, Arbitrary Units; OD, Optical Density. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
Article Snippet: Antinuclear antibodies (ANA) levels were assessed using ANA mouse bioassay kits (USBiologicals, Swampscott, MA, USA); total IgG levels were assessed with mouse IgG Total Ready-SET-Go kits (Affimetrix, Vienna, Austria); anti-double strand DNA (anti-dsDNA) levels were assessed with
Techniques: Enzyme-linked Immunosorbent Assay, Injection, Microarray, Immunopeptidomics, Transplantation Assay